OWLS 2012 - Italy
Transkript
OWLS 2012 - Italy
OWLS 2012 ORGANISING COMMITTEE Alberto Diaspro, Istituto Italiano di Tecnologia and University of Genoa, Italy Francesco Baldini, National Research Council, Florence, Italy Paolo Bianchini, Istituto Italiano di Tecnologia, Genoa, Italy Francesca Cella Zanacchi, Istituto Italiano di Tecnologia, Genova, Italy Valeria Delle Cave,Istituto Italiano di Tecnologia, Genova, Italy Zeno Lavagnino, Istituto Italiano di Tecnologia and University of Genoa, Italy Mattia Pesce, Istituto Italiano di Tecnologia, Genova, Italy Manuela Rigano, University of Genova, Italy Manuela Salvatori, Istituto Italiano di Tecnologia, Genova, Italy Federico Tixi, Istituto Italiano di Tecnologia, Genova, Italy Cesare Usai, National Research Council, Genova, Italy PROGRAM Alberto Diaspro, Istituto Italiano di Tecnologia and University of Genoa, Italy Silvio Aime, University of Turin, Italy Francesco Baldini, National Research Council, Florence, Italy Gert von Bally, University of Muenster, Germany Paolo Bianchini, Istituto Italiano di Tecnologia, Genoa, Italy Francesca Cagnoni, Istituto Italiano di Tecnologia, Genova, Italy Halina Rubenstein-Dunlop, University of Queensland, Australia Hans-Jochen Foth, Technical University of Kaiserslauter, Germany Min Gu, Swinburne University, Australia Partha Pratim Mondal, Indian Institute of Science, Bangalore, India Stephen Morgan, University of Nottingham, UK Colin JR Sheppard, National University of Singapore, Singapore Cesare Usai, National Research Council, Genova, Italy OWLS • Genoa, 4-6 July 2012 • Program • Page 1 Exhibit Area 2nd Floor G H I L M N Nikon Instruments Spa IIT - Istituto Italiano di Tecnologia / LANIR.EU Physik Instrumente (PI) S.r.l. HAMAMATSU PHOTONICS ITALIA Till Photonics GmbH Fianium Limited Page 2 • Program • OWLS • Genoa, 4-6 July 201 2 3rd Floor A B C D E-F OLYMPUS ITALIA SPA VUTARA Inc. TOPTICA Photonics AG Bitplane AG Leica Microsystems Srl OWLS • Genoa, 4-6 July 2012 • Program • Page 3 Page 4 • Program • OWLS • Genoa, 4-6 July 201 2 OWLS 2012 Tuesday, July 3rd - 7.00pm Inaugural Ceremony Sala del Maggior Consiglio, Palazzo Ducale www.palazzoducale.genova.it Alberto Diaspro, OWLS President Roberto Cingolani, Scientific Director, Istituto Italiano di Tecnologia “Lettura Magistrale” Scott E. Fraser, Caltech, CA Imaging the Molecular and Cellular Dynamics of Embryogenesis Welcome Cocktail kindly offered by Camera di Commercio di Genova Leica Microsystems Bitplane OWLS • Genoa, 4-6 July 2012 • Program • Page 5 Wednesday, July 4th 9.00 - 9.30 Magazzini del Cotone - Congress Center http://www.portoantico.it Scirocco-Libeccio Room OWLS 2012 Opening Lecture Karsten Konig, Saarbrucken University, Germany Clinical Multimodal Hybrid Multiphoton/CARS Tomography of In Vivo Human Skin Chair: A. Diaspro Parallel Sessions 9.30 - 10.00 Scirocco - Libeccio Room David Sampson University of Western Australia, Australia Optical elastography - high-resolution imaging of tissue mechanical properties. Ponente Room Gertrude Bunt University of Goettingen, Germany Visualization of multiple coupled signaling events in a single cell by fluorescence Chair: G. Scarcelli lifetime pattern analysis. Chair: R. Bizzarri 10.00 - 10.45 Optical application to biomedicine Advanced imaging techniques I Chair: G.Scarcelli Chair: R. Bizzarri Andrea Curatolo University of Western Australia, Australia Portable 3D optical coherence elastography for applying microscopic imaging of tissue mechanical properties in the clinic. Barbara Storti NEST, Pisa Italy Multiplexing correlation spectroscopy and resonance energy transfer unveils dynamical interactions between transient receptor potential vanilloid 1 (trpv1) and George A. Stanciu University Politehnica Bucharest, Romania microtubules at nanoscale in living cells. Electrical charge on the hydroxyapatite Dominika O. Trembecka-Lucas surface investigated by laser scanning Jagiellonian Universit. Poland microscopy. Interaction between HP1 dimer and PCNA in DNA replication and repair, as S. Beke revealed by bimolecular fluorescence Istituto Italiano di Tecnologia (IIT), Gencomplementation assay (BiFC), and ova, Italy Excimer laser-based stereolithography FRET/FLIM. for rapid fabrication of rigid biodegrad- Simona Rodighiero able photopolymer scaffolds for tissue Fondazione Filarete, Milan, Italy engineering. FRET study of 4.1R, ICln and actin interactions: a link between cell morphology and cell proliferation. Page 6 • Program • OWLS • Genoa, 4-6 July 201 2 10.45 - 11.15 Giuliano Scarcelli Harvard Medical School, USA Brillouin microscopy to study the bio-mechanical properties of cornea and lens. Ranieri Bizzarri National Research Council, Italy Novel probes and strategies for high-resolution fluorescence imaging in living cells. Chair: D.Sampson Chair: G.Bunt 11.15 - 11.45 Coffee break 11.45 - 12.15 Scirocco-Libeccio Room Monika Ritsch-Marte Medizinische Universität Innsbruck, Austria Applications of spatial light modulators in biophotonics: from optical tweezers to advanced microscopy. Chair: F.Difato Parallel Sessions 12.45 - 13.30 Scirocco - Libeccio Room Ponente Room Plasmonics Advanced imaging techniques II Chair: E. Di Fabrizio Chair: A. Periasamy Emanuela Esposito Antonella Battisti CNR-ICIB Napoli, Italy NEST, Pisa, Italy Lab-on-Fiber Technology: Nanophoton- Intracellular pH measurements made ics Within Optical Fibers. simple by genetically-encoded fluorescent probes and phasor approach to Fang Liu FLIM. Tsinghua University, Beijing Integrated Bio-Sensor based on Hybrid Ottavia Golfetto Coupler with Surface Plasmon Polariton University of California, Irvine, California and Dielectric Waveguide. Laurdan fluorescence lifetime as a biosensor for membrane fluidity. Ranjeet Kumar Indian Institute of Technology New Delhi, India Clustering of Au-NPs near plasmon excitation and enhanced scattering based in-vitro detection of Ehrlich ascites carcinoma cells. Deo R. Singh University of Wisconsin Milwaukee, USA Quaternary structure of wild type and mutated rhodopsin at room and body temperature using spectral FRET. 13.30 - 15.30 Lunch, posters, exhibition, Job corner. Company Presentations 14.00 - 15.00 Scirocco - Libeccio Room Olga Levai Leica Microsystems Opening the gate to Super-Resolution. Ponente Room Maria Marosvölgyi Till Photonics Combining advanced fluorescence imaging techniques on the same living sample: spinning disk confocal, TIRF, FRAP(PA) and FRET. OWLS • Genoa, 4-6 July 2012 • Program • Page 7 15.30 - 16.00 Scirocco - Libeccio Room Anthony Watts Oxford University, UK Realizing bio-imaging potential: Enhancing bacteriorhodopsin-mediated molecular scale, wavelength-selective photoswitching. Chair: A. Periasamy Parallel Sessions 16.00 - 16.30 Scirocco - Libeccio Room Ilaria Testa Max Planck Institute Goettingen, Germany RESOLFT Nanoscopy in Life Sciences: unraveling the mysteries with low light levels Ponente Room Arthur Chiou National Yang-Ming University, Taiwan (R.O.C.) Optical Tweezers Based BioMicrorheology Chair: G. Vicidomini 16.30 - 17.15 Single molecule and super-resolution Optical Tweezers Chair: I.Testa Chair: D. Cojoc Chair: A. Chiou Paolo Annibale Francesco Difato EPFL, Lausanne, Switzerland Istituto Italiano di Tecnologia, Genova, Towards accurate dual color PALM im- Italy aging. Position and Force-clamp interferometric tracking of an optically trapped probe to Francesca Cella Zanacchi study cytoskeleton dynamics of develIstituto Italiano di Tecnologia, Genova, oping mouse neuron. Italy Super-resolution microscopy of large Pasquale Bianco biological samples within a light sheet Università degli Studi di Firenze, Italy based architecture. The energy landscape of DNA overstretching transition determined with a Davide Mazza Dual Laser Optical Tweezers under force Fondazione S.Raffaele, Milano, Italy Measurement of binding in living cells by clamp. single molecule tracking. A benchmark Dan Cojoc for protein/scaffold interactions. Materials Technology Institute, Trieste, Italy Local delivery of small numbers of attractant and repulsive guidance molecules to neuronal cells by optical manipulation techniques. 17.15 - 17.45 Mike Heilemann Maria Luisa Calvo Padilla Wuerzburg University, Germany Universidad Complutense de Madrid, Spain Quantitative Single Molecule Biology Holography and its applications. with Photoswitchable Fluorophores. Chair: A.Chiou Chair: I.Testa Page 8 • Program • OWLS • Genoa, 4-6 July 201 2 17:45 - 18:15 Coffee break 18:15 - 18:45 Scirocco - Libeccio Room Brad Amos Cambridge, UK A laser-scanning microscope for optical sectioning of large-volume specimens such as mouse embryos using a Mesolens. Chair: C.J.R. Sheppard Parallel Sessions 18.45 - 19.30 Scirocco - Libeccio Room Ponente Room Optical tomography Optical application to biomedicine II Chair: C.J.R. Sheppard Chair: K. Braeckmans Stephen P Morgan University Park, Nottingham, UK Pulsed ultrasound modulated optical tomography of tissue Maria Pogorelova Moscow State University, Moscow, Russian Federation Optical microtomography of early mouse embryo with laser scanning microscopy Ludovico Silvestri University of Florence, Italy Whole-brain neuroanatomy on the micron-scale with confocal light sheet microscopy 19.30-20.00 Martin J. Booth Oxford University, UK Adaptive optics for high resolution biological microscopy Wei Zhang Tsinghua University, Beijing, China Trace level multi-gas sensing in mid-infrared hollow-core Bragg fiber supporting multi-wavelength transmission Tadelech Laser-Induced Breakdown and X-Ray Fluorescence Spectroscopic Techniques in Detection of Elements in Tea Leaves Tewfik Soulimane University of Limerick, Ireland Photosterilisable Textiles for Improved Hospital Hygiene Dayong Jin Macquarie University, Sydney, NSW 2109, Australia Novel coding and decoding in suspension Chair: C.J.R. Sheppard arrays for accelerated biomolecular discovery and personalized medicine. Shan Shan Kou EPFL, Lausanne, CH-1015 Switzerland Three-dimensional (3D) Monte Carlo Simulation of Born’s Diffractions for Cellular Analysis OWLS • Genoa, 4-6 July 2012 • Program • Page 9 Thursday, July 5th 8.30 - 9.00 Scirocco - Libeccio Room RMS Lecture Tony Wilson Oxford University, UK Making Light Work in Microscopy. Chair: F. Brakenhoff Parallel Sessions 9.00 - 9.30 Scirocco - Libeccio Room Martin Oheim Université Paris Descartes, France Non-evanescent excitation light in objective-type TIRF microscopy limits to quantitative imaging and super-resolution microscopy. Ponente Room Amitabha Chattopadhyay Centre for Cellular and Molecular Biology Hyderabad, India Organization and Dynamics of the Serotonin-1A Receptor in Live Cells using Fluorescence Microscopy. Chair: J. Bewersdorf 9.30 - 10.15 STED microscopy I Chair: S. Maiti Advanced Imaging Techniques III Chair: J. Bewersdorf Chair: A. Chattopadhyay Giuseppe Vicidomini Istituto Italiano di Tecnologia, Genoa, Italy Towards Low-Intensity STED Microscopy by Time Gating. Lorena Benedetti University of Milan, Italy Morphological features of 4.1R overexpressing cells characterized with Correlative Light-Scanning Electron Microscopy and observed with gated-STED nanoscopy. Cosimo Arnesano University of California Irvine, Irvine, California Digital Parallel Frequency-Domain Spectroscopy for Tissue Imaging. Stefano Coppola Sapienza - University of Rome, Italy Intracellular trafficking of multicomponent lipoplexes: a fluorescence microscopy study. Tilman Rosales National Institutes of Health, USA Instrumentation for super-resolution using new low power probes. 10.15 - 10.45 Joerg Bewersdorf Yale University, USA New Approaches in STED Microscopy: TIRF and Phase Retrievaly. Anna Letizia Allegra Mascaro University of Florence, Italy In vivo imaging of climbing fibers reactive plasticity after laser axotomy. Sudipta Maiti Tata Institute of Fundamental Research, India Protein misfolding versus aggregation: Chair: M. Oheim following the early events with an ultrasensitive fluorescence tool. Chair: A. Chattopadhyay Page 10 • Program • OWLS • Genoa, 4-6 July 201 2 OWLS • Genoa, 4-6 July 2012 • Program • Page 10 10:45 - 11:15 Coffee break 11.15 - 11.45 Scirocco - Libeccio Room Ernst H.K. Stelzer Goethe University Frankfurt , Germany From the ultramikroskop to theta microscopy to tetrahedral microscopy to light sheet-based fluorescence microscopy. Chair: F. Cella Zanacchi Parallel Sessions 11.45 - 12.30 Scirocco - Libeccio Room Ponente Room Single Particle Tracking Label Free Techniques Chair: Z. Lavagnino Chair: P. O’Shea Hendrik Deschout Ghent University, Belgium On-chip sheet illumination for single-particle tracking measurements in bio-fluids. Katrien Forier Ghent University, Belgium Single particle tracking microscopy: Towards nanomedicines for the treatment of chronic pulmonary infections in cystic fibrosis patients. Christelle Rosazza University of Konstanz, Germany Single Particle Tracking of DNA in cells delivered by electroporation. 12.30 - 13.00 Kevin Braeckmans University of Ghent, Belgium Sizing Nanomatter in Biological Fluids by Fluorescence Single Particle Tracking. Francesca Romana Bertani National Research Council, Rome, Italy Hyperspectroscopic confocal reflectance imaging of cultured cells. Alessandro Candiani University of Parma, Italy Label-free DNA sensor based on optical fiber grating devices. Dan Cojoc National Research Council, Trieste, Italy Secondary speckle sensing microscopy for fast detection of malaria. Paul O’ Shea University of Nottingham, UK Imaging specific proteins in cells; time-resolved dynamics and disposition Chair: Z. Lavagnino without GFP. Chair: A. Chattopadhyay 13.00 - 15.00 Lunch, posters, exhibition, Job corner. Company Presentations 14.00-15.00 Scirocco-Libeccio Room Ponente Room Luciano Lucas Alessandro Cometta Bitplane Zeiss Imaris: the 3D/4D image analysis soft- A super-resolution close-up view: from ware SIM to PALM Page 11 • Program • OWLS • Genoa, 4-6 July 201 2 OWLS • Genoa, 4-6 July 2012 • Program • Page 11 15.00 - 15.30 Scirocco-Libeccio Room Colin Sheppard National University of SIngapore, Singapore and Istituto Italiano di Tecnologia, Italy Imaging in the phase contrast microscope. Chair: P.Saggau 15.30 - 16.00 Gail McConnell University of Strathclyde, UK Promising new wavelengths for multiphoton laser scanning microscopy: thinking outside the Ti:Sapphire Chair: P.Saggau Parallel Sessions 16.00 - 17.30 Scirocco - Libeccio Room Ponente Room Non Linear/Multiphoton Microscopy Fluorescent Probes/Sensors Chair: G. McConnell Chair: F. Brandi Giulia Borile Komal Bagga University of Padova, Italy Istituto Italiano di Tecnologia, Genoa, Italy Multiphoton fluorescence Ca2+ imaging Laser-based generation of luminescent in acute cardiac slices. bio-nanomarkers for cell imaging. Albrecht Haase Ilaria De Santo University of Trento,Italy Istituto Italiano di Tecnologia, Naples, Italy In-vivo morpho-functional imaging of Understanding Drug Localization in Doxthe honeybee brain . orubicin Loaded TiO2 Nanotube Arrays through Fluorescence Correlation SpecB. Leshem troscopy analysis. Paris Descartes University Temporally focused illumination is inher- Joanna L Richens ently robust against scattering. University of Nottingham, UK Plasmonic-Based Biosensors in Disease Leonardo Sacconi National Research Council, Florence, Italy Diagnosis. Optical recording of intracellular action Shu-Han Wu potential propagation in cardiac myo- National Yang-Ming University, Taipei, cytes. Taiwan Dynamic Study of Cell Secretion using Godofredo Bautista Microfluidic Traps and Gold Nanoslits Tampere University of Technology, FinBased Surface Plasmon Sensors. land Wide field third-harmonic generation Milena Stephan microscopy of turbid media. Georg-August University of Goettingen, Germany Neeven A. Hosny Combining Fluorescence Microscopy Imperial College London, Great Britain Viscosity imaging of lipid-based micro- with Reflectometry: Development of a bubbles using fluorescence lifetime im- Biomembrane Sensor. aging (FLIM) of molecular rotors. Page 12 • Program • OWLS • Genoa, 4-6 July 201 2 OWLS • Genoa, 4-6 July 2012 • Program • Page 12 17.30 - 18.00 Scirocco - Libeccio Room Francesco S. Pavone European Laboratory for Non Linear Spectroscopy, University of Florence In Vivo Reactive Neural Plasticity Investigation by means of Correlative Light-Electron Microscopy. Chair: P. Bianchini 18.00 - 19.15 OWLS Assembly with Coffee break 20.30 Social Dinner Villa dello Zerbino http://www.villalozerbino.it/ OWLS • Genoa, 4-6 July 2012 • Program • Page 13 Friday, July 6th 9.00 - 9.30 Scirocco - Libeccio Room Enrico Gratton University of California Irvine, US Super-resolution by feedback imaging: mechanisms of translocation through the nuclear pore complex. Chair: P. Bianchini 9.30 - 10.00 Daniel Farkas Cedars-Sinai Medical Center, USA Multimode Optical Molecular Imaging of Disease for Better Diagnosis and Treatment. Chair: P. Bianchini Parallel Sessions 10.00 - 10.45 Scirocco - Libeccio Room Ponente Room Amyloid Studies Optics in the environmental science Chair: C. Canale Chair: F. Baldini Valeria Musi Ecole Polytechnique Fédérale de Lausanne, Switzerland Bloch surface wave optical platform for sensing the aggregation kinetics of amyloid beta peptides. Towards the early detection of Alzheimer’s disease. Malgorzata Biczysko Istituto Italiano di Tecnologia, Pisa, Italy Simulation of optical spectra line-shapes for biomolecules in natural environmental conditions. Niels Zijlstra University of Twente, Netherlands Establishing the composition of alpha-synuclein amyloid oligomers using single-molecule photobleaching. Dayong Jin Macquarie University, Sydney, Australia Tuning the Rare-earth Nanoparticles for Background-free Biosensing. Sanjaya Thilakarathne Saitama University, Japan Bidyut Sarkar Ultra short term growth dynamics of Tata Institute of Fundamental Research, Soybean and Radish under ozone stress Mumbai, India using Statistical Interferometry TechLabel free dopamine imaging with multi- nique - a comparison with conventional photon ultraviolet microscopy. ozone assessment measures. 10.45 - 11.15 Coffee break 11.15 - 11.45 Scirocco - Libeccio Room Nicolai Urban Max Planck Institute Goettingen, Germany Dynamic superresolution microscopy deep inside living brain tissue. Chair: P. Bianchini Page 14 • Program • OWLS • Genoa, 4-6 July 201 2 Parallel Sessions 11.45 - 12.30 Scirocco - Libeccio Room Ponente Room Sted Microscopy II Novel Techniques Chair: B. Harke Chair: E. Gratton Paolo Bianchini Cristophe Silien Istituto Italiano di Tecnologia, Genoa, Italy University of Limerick, England Single wavelength 2PE-STED nanoscopy. Chemical Imaging with Super Resolution Infra-Red Absorption. Brian R. Rankin Mobius Photonics, CA, USA A new laser for STED P. Nandakumar BITS Pilani K. K. Birla Goa Campus, India Laser scanning photothermal microscopy. Jay R. Knutson National Institutes of Health, USA William M. Whelan A new class of low-power probes for mul- Atlantic Veterinary College, Canada ticolor nanoscopy. Frequency spectrum analysis in photoacoustic tissue characterization. 12.30 - 13.00 Peter Saggau Ari T. Friberg Baylor College, Texas, USA National Institute of Advanced Industrial Advanced Optical Imaging for Re- Science and Technology (AIST), Japan verse-Engineering the Brain Classical Ghost Imaging of Phase Objects and through Phase Aberrations Chair: B. Harke Martijn Stopel University of Twente, Netherlands Room temperature excitation spectroscopy of single quantum dots 13.00 - 15.00 Lunch, posters, exhibition, Job corner. 15.00 - 15.30 Scirocco - Libeccio Room Ponente Room Maria Garcia Parajo ICFO- Institut de Ciences Fotonique Barcelona, Spain Cell surface nanoscopy using single molecule optical nanotools Chair: M. Heilemann 15.30 - 16.00 EBSA Lecture Andreas Zumbusch Universitat Konstanz, Germany Coherent anti-Stokes Raman Scattering microscopy of intracellular dynamics Chair: M. Heilemann OWLS • Genoa, 4-6 July 2012 • Program • Page 15 Parallel Sessions 16.00 - 17.30 Scirocco - Libeccio Room Nanoparticles & Smart Materials Ponente Room Force Microscopy Chair: M. Lorenzoni Chair: C. Canale Athanassia Athanassiou Istituto Italiano di Tecnologia, Genoa, Italy Nanocomposite surfaces with photoinduced wettability gradient for directional growth of cells. Marco Capitanio European Laboratory for Non-linear Spectroscopy, Sesto Fiorentino, Italy Ultrafast force-clamp spectroscopy of single biomolecules Francesco Baldini National Research Council, Floresnce, Italy PMMA-nanoparticles for molecular beacon delivery in cells for survivin mRNA detection. Josè L.Toca-Herrera University of Natural resources and Life Sciences Wien, Austria Delivering mechanical and interfacial sample properties by combining optical and atomic force microcopies Despina Fragouli Istituto Italiano di Tecnologia, Lecce, Italy Rendering cellulose sheets antibacterial using silver nanoparticles formed by a laser ablation technique. Jenu Varghese Chacko Istituto Italiano di Tecnologia, Genoa, Italy AFM STED: Targeted probing with high spatial precision Alemu Kebede Addis Ababa University, Etiopia Controlled Synthesis, Characterization and Application of Iron Oxide Nanoparticles for Oral Delivery of Insulin. Raman Spectroscopy Laura D’Alfonso Università degli Studi di Milano Bicocca, Milan, Italy Intracellular diffusion of Gold Branched Nanoparticles. Italy Modulated Raman spectroscopy: a promising biophotonics tool for early cancer diagnosis Chair: R. Proietti Riccardo Cicchi European Laboratory for Non-linear Spectroscopy, Sesto Fiorentino, Italy Enrico Rampazzo A combined fluorescence-Raman specUniversità di Bologna, Italy troscopic probe for the diagnosis of Fluorescent Silica-Core/PEG-Shell Nano- melanocytic lesions particles with Tunable emission for Imag- Anna Chiara De Luca ing and Labeling. Institute of Protein Biochemistry, Naples, Page 16 • Program • OWLS • Genoa, 4-6 July 201 2 Nicholas Smith Osaka University, Japan Raman imaging and analysis: from quantification of cellular morphology to RNA structure and intramolecular interactions. 17.30 - 18.00 Closing Ceremony Scirocco-Libeccio Room OWLS 2012 Closing Lecture Satoshi Kawata Osaka University, Japan Plasmons, Raman, nano and 3D, all in together. Chair: A. Diaspro 18.00 - 18.30 Poster prizes Sponsored by OWLS, Université Laval Québec, Camera di Commercio di Genova Farewell with Focaccia and white wine Sponsored by Camera di Commercio, Leica Microsystems, Bitplane Friday, July 6 2012 8.30pm City Lights by LAMBS www.lambs.it Piazza De Ferrari, Genova (De Ferrari Square) Saturday, July 7 2012 Full Day Cinque Terre Tour www.cinqueterre.it OWLS • Genoa, 4-6 July 2012 • Program • Page 17 OWLS 2012 Poster Session “An insight of NoBoDys PerFect” Exhibition P1 - P6 Page P1 Gaser N. Abdelrasoul1,2 , Raffaella Magrassi3, Marco Scotto d’Abbusco1, Alberto 120 Diaspro1, 2 1 Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy 2 Department of Physics University of Genova, via Balbi 5, 16126 Genova, Italy 3 National Research Council (CNR) - Institute of Marine Sciences (ISMAR), Genova, Italy 2PE Imaging of Cancer Cells Using PEGylated Gold Nanorods as Biocompatible Contrast Agent. P2 Giulio Caracciolo1, Daniela Pozzi1, Anna Laura Capriotti2, Chiara Cavaliere2, Francesco Cardarelli3, Angelo Bifone3, Giuseppe Bardi3, Fabrizio Salomone3 and Aldo Laganà2 123 1 Department of Molecular Medicine, ‘Sapienza’ University of Rome, Viale Regina Elena, 324, 00161, Rome, Italy 2 Department of Chemistry, ‘Sapienza’ University of Rome, P.le A. Moro 5, 00185, Rome, Italy, 3 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa, Italy Protein corona controls the uptake delivery mechanisms of lipid gene vectors in cancer cell P3 Tatjana Dramićanin, Lea Lenhardt, Ivana Zeković, Nasta Tanić and Miroslav D. Dramićanin 128 Vinča Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade, Serbia Breast Cancer Detection by Support Vector Machine Classification of Synchronous Fluorescence Spectroscopy Data P4 Ivana Zekovića, Lea Lehnardta*, Tatjana Dramićanina, Miroslav D. Dramićanina a Vinča Institute of Nuclear Sciences, University of Belgrade, PO Box 522, 11001 Belgrade, Serbia Application of Supervised Self-Organizing Maps in Skin Melanoma Diagnosis by Total Synchronous Fluorescence Spectroscopy P5 Elisabetta M. Caspani, Philip H. Crossley, Carolina Redondo-Garcia, Salvador Martinez 144 * Laboratory of Experimental Embryology, Instituto de Neurociencias UMH-CSIC, Alicante, Spain Malignopodium: a new player in glioma progression P6 Roberto Cighettia, Matteo Piazzaa, Valentina Calabresea, Gaetana Damorea and Francesco Peria a Università degli Studi di Milano-Bicocca, Piazza della Scienza, 2 – 20126 – Milano, Italy New fluorescent molecular tools to investigate LPS recognition Page 18 • Program • OWLS • Genoa, 4-6 July 201 2 124 P16 - P30 P16 Hanna Beckera,2, Amy Gladfelter3, Rudolf Oldenbourg1 Page 121 1 Marine Biological Laboratory, Woods Hole, Massachusetts, USA 2 Universität Konstanz, Konstanz, Germany 3 Dartmouth College, Hanover, New Hampshire, USA Quantitative and high resolution imaging of fluorescence anisotropy is shedding light on septin architecture in the yeast bud neck P17 Iván, Coto Hernández1,2, Giuseppe, Vicidomini1, Alberto, Diaspro1,2 125 1 Istituto Italiano di Tecnologia, Genova, Italia 2 Universita Degli Studi di Genova, Genova, Italia Generation Of Super Continuum Source And Its Application In Nanoscopy P18 William F. Falco, Joelson Fernandes, Evaristo A. Falcão, Anderson R.L. Caires 130 Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil Effects on Photosynthetic Activity of Plants Induced by Silver Nanoparticles: Monitoring by Chlorophyll Fluorescence P19 Joelson Fernandes1, William F. Falco1, Samuel L. Oliveira2, Anderson R.L. Caires1 131 1 Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil 2 Grupo de Óptica e Fotônica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil Changes on Photosynthetic Activity of Herbicide-tolerant Soybean Plants Induced by Glyphosate P20 Jenni Karttunen1, Sari Mäntynen1, Heli Lehtivuori1, Janne Ihalainen1, Teemu Ihalainen1, Jaana K. H. Bamford1 and Hanna M. Oksanen2 138 1 Department of Biological and Environmental Science and Nanoscience Center, PO Box 35, 40014 University of Jyväskylä, Jyväskylä, Finland 2 Institute of Biotechnology and Department of Biosciences, PO Box 56, 00014 University of Helsinki, Helsinki, Finland Protein Localization and Interactions in Bacteria Studied with Confocal Microscopy and Fluorescence Lifetime P21 Volkan KILIÇ, Gözde AYDOĞAN KILIÇ, Ahmet ÖZATA 140 Anadolu University, Faculty of Science, Department of Biology, 26470, Eskişehir, TURKEY Light microscopic pathology in Common carp ( Cyprinus carpio L.,1758) from Sakarya River (TURKEY) P22 Lea Lenhardt1*, Ivana Zeković1, Tatjana Dramićanin1, Živoslav Tešić2, Dušanka Milojković-Opsenica2, Miroslav D. Dramićanin1 143 1 Vinča Institute of Nuclear Sciences, University of Belgrade, PO Box 522, 11001 Belgrade, Serbia 2 Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia. Assessment of Fluorescence and Infrared Spectroscopy Capabilities for the Determination of Botanical Origin of Serbian Unifloral Honey OWLS • Genoa, 4-6 July 2012 • Program • Page 19 P16 - P30 P23 Daniela Pozzi1, Giulio Caracciolo1, Francesco Cardarelli2, Angelo Bifone2, Heinz Amenitsch3 Page 152 1 Department of Molecular Medicine, “Sapienza” University of Rome, Viale Regina Elena, 324, 00161, Rome, Italy 2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa, Italy 3 Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences, Schmiedelstrasse 6, A-8042 Graz, Austria Confocal microscopy makes visible cholesterol-mediated gene delivery mechanisms P24 Liliia Rohoza, Tatyana Dyubko, Sergey Galchenko, Boris Sandomirsky Institute for Problems of Cryobiology and Cryomedicine fo the NAS of Ukraine, Kharkiv Spectrofluorimetric investigation of interactions of heart peptides with blood serum albumin P25 Sebastian Tannert1, Peter Kapusta1, Alexander Glatz1, Christian Litwinski1, Uwe Ortmann1, Rainer Erdmann1, Katrin Hoffmann2, Ute Resch-Genger2 153 154 1 PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, www.picoquant.com 2 Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Str. 11, 12489 Berlin, Germany) On the spectral sensitivity calibration of fluorescence spectrometers: Extension to the NIR, polarization and grating effects P26 Anatoliy Tatarets1*, Yevgeniy Povrozin1, Olena Obukhova1, Iryna Fedyunyayeva1, Ewald Terpetschnig2, Leonid Patsenker1,2 155 1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001 Kharkiv (Ukraine) 2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA, Novel Squaraine Probes for Proteins and Fluorescence Imaging Applications P27 Francesco Urciuolo1, Giorgia Imparato1, Costantino Casale1, Fabio Formiggini1, Paolo Netti1,2 1 Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci n, 53 80125 Napoli 2 Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, P. Le Tecchio 80, 80125 Naples, Italy Coupling Engineered Living Tissue with Multiphoton Tomography : towards in vitro understating of biological phenomena Page 20 • Program • OWLS • Genoa, 4-6 July 201 2 156 P31 - P45 P31 William Dallari, Marco Allione, Elena Samoylova, Marco Scotto d’Abbusco, Alberto Diaspro Page 126 Nanophysics, Istituto Italiano di Tecnologia, via Morego, 30, 16163 Genova Permanent and rewritable 3D optical data storage through multi-photon absorption P32 Silvia Galiani1, Benjamin Harke1, Giuseppe Vicidomini1, Gabriele Lignani2, Fabio Benfenati2, Hanako Tsushima2, Evelina Chieregatti2, Paolo Bianchini1, Alberto Diaspro1 133 1Nanophysics, Istituto Italiano di Tecnologia, Genoa, Italy 2Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genoa, Italy Optimization of a STED nanoscope P33 Manuel Gunkela, Nina Beila, Jürgen Benekea, Slawek Mazura, Jürgen Reymanna, Bastian Schumachera and Holger Erflea 135 a Viroquant-CellNetworks RNAi Screening Facility, BioQuant, University of Heidelberg, Germany Automated microscopy in RNAi screening experiments P34 Timo Hakkarainen1, Tero Setälä1, Ari T. Friberg1,2,3 136 Near-Field Imaging of Interacting Nano-Particles P35 Christian Jüngst*and Andreas Zumbusch 137 1 Department of Applied Physics, Aalto University, Box 13500, FI-00076 Aalto, Finland 2 Department of Physics and Mathematics, University of Eastern Finland, Box 111, FI-80101 Joensuu, Finland 3 Department of Microelectronics and Applied Physics, Royal Institute of Technology (KTH), Electrum 229, SE-164 40 Kista, Sweden Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany Label-free imaging of Lipid Droplets in living cells P36 Marcelle Koenig1, Steffen Ruettinger1, Sebastian Tannert1, Thomas Schoenau1, Christian Litwinski1, Olaf Schulz2, Michael Wahl1, Marcus Sackrow1, Kristian Lauritsen1, Felix Koberling1, Robert Ros2, and Rainer Erdmann1 141 1 PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, info@picoquant.com 2 Arizona State University, Lab Robert Ros, Physics Department, Tempe, AZ 85287-1504, USA Pushing the boundaries of single molecule detection P37 Zeno Lavagninoa1, Francesca Cella Zanacchi1, Alberto Diaspro1,2 142 Thick Sample imaging and scattering effects: a phantom sample based characterization of Single Plane Illumination Microscopy performances P38 Aimar Namma2, Andres Arend1, Marina Aunapuua,2 146 1 Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30,16163 Genova, Italia 2 LAMBS- Dept. of Physics – University of Genoa, Via Dodecaneso 33, 16153 Genova, Italia 1 Department of Anatomy, University of Tartu, Ravila 19, 50411 Tartu, Estonia 2 Department of Veterinary Medicine, Estonian University of Life Sciences, Fr Kreutzwaldi 62, 51014 Tartu Computer assisted image analysis in the detection of expression of bone morphogenetic proteins in the neural tube of human embryos P39 Marco Zanella1†, Luca Ceseracciu2†, Roberta Ruffilli1, Alberto Diaspro2, Liberato Manna1 158 1 Nanochemistry, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy 2 Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy Polarized plasmonic absorption in micrometer long copper(I) selenide nanowires OWLS • Genoa, 4-6 July 2012 • Program • Page 21 P46 - P60 P46 Abebe Belay Adama Science and Technology University, School of Natural Sciences, Department of Physics, P.O.Box, 1888, Adama, Ethiopia, Page 122 The Hetero-Association of Caffeine (CA) with 5-Caffeoyl quinic Acid (5-CQA) and Ethidium Bromide (EB) in Aqueous Solution P47 Mariagrazia Di Luca1*, Francesco Cardarelli2, Ranieri Bizzarri 1,3 127 Fluorescent recombinant virus like particles as nano-carriers of sensors P48 Sabato Fusco, Valeria Panzetta, Paolo Antonio Netti 132 1 NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12 - 56127 Pisa 2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa 3 Istituto di Biofisica - CNR, via Moruzzi 1, 56124 Pisa Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia Largo Barsanti e Matteucci, 53 80125 Napoli Cell Mechanical Characterization by Particle Tracking Microrheology in Optical Microscopy P49 Gallus Lorenzo1*, Ferrando Sara1, Gambardella Chiara1, Amaroli Andrea1, Faimali Marco2, Veronica Piazza2, Bianchini Paolo3, Masini Maria Angela1 134 1 Dip. di Scienze della Terra, dell’Ambiente e della Vita (DiSTAV), Univ. di Genova 2 Ist. di Scienze Marine, ICMM, CNR, Area di Genova 3 Dipartimento di Nanofisica, Istituto Italiano di Tecnologia Genova Aquaporins in cyprid of barnacle Balanus amphitrite (=Amphibalanus amphitrite) (Cirripedia, Crustacea) P50 Gözde Aydoağan KILIÇ, Volkan KILIÇ, Ahmet ÖZATA Anadolu University, Faculty of Science, Department of Biology, 26470, Eskişehir, TURKEY Autometallographic localization of heavy metals and immunoperoxidase detection of metallothionein induction in Common carp (Cyprinus carpio L.1758) from Sakarya River (TURKEY) P51 Keurison F. Magalhães1,3, Anderson R.L. Caires1*, Margarete S. Silva2, Samuel L. Oliveira3 139 145 1 Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil 2 Grupo de Estudo e Desenvolvimento de Materiais, Universidade Estadual de Mato Grosso do Sul, Dourados, MS,Brazil 3 Grupo de Óptica e Fotônica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil The Role of Fatty Acid Methyl Esters on Optical Properties of Biodiesel P52 Olena Obukhova1*, Anatoliy Tatarets1, Olga Kolosova1, Iryna Fedyunyayeva1, Leonid Patsenker1,2, Ewald Terpetschnig2 1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001 Kharkiv (Ukraine) 2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA Highly Photostable Biomedical Markers Based on Thiosquarylium Dyes Page 22 • Program • OWLS • Genoa, 4-6 July 201 2 147 P53 Silvio Panettieri1 Giovanni Signore2 Ranieri Bizzarri2 148 1 NEST, Istituto Nanoscienze - CNR, Piazza S. Silvestro 12, 56127 Pisa 2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa Synthesis, stereochemical characterization, and photophysical features of an innovative 3-styryl coumarin acting as polarity/viscosity sensor for bioanalytical use P54 Leonid Patsenker1,2*, Ewald Terpetschnig2, Anatoliy Tatarets1, Olena Obukhova1, Olga Kolosova1, Larysa Markova1, Inna Yermolenko1 149 1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001 Kharkiv (Ukraine). 2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA, Next Generation Fluorescent Dyes for Biomedical Applications P55 Alessia Petrelli, Claudio Canale, Alberto Diaspro and Silvia Dante Nanophysics Department, Fondazione Istituto Italiano di Tecnologia, Genova (Italy) Quantitative evaluation of cell adhesion on patterned surfaces P56 Ferruccio Pisanello1,2*, Luigi Martiradonna1, Godefroy Leménager3, Alberto Bramati3, Massimo De Vittorio1,4 150 151 1 Center for Bio-Molecular Nanotechnologies @UNILE, Istituto Italiano di Tecnologia, 73010 Arnesano (Lecce), Italy 2 Center for Neuroscience and Cognitive Systems @UNITN, Istituto Italiano di Tecnologia, 38068 Rovereto (TN), Italy 3 Laboratoire Kastler Brossel, Université Pierre et Marie Curie, CNRS UMR 8552, Ecole Normale Supérieure, 75005 Paris, France 4 National Nanotechnology Laboratory, CNR-‐Nano, Università del Salento, Dipartimento Ingegneria dell’innovazione, 73100 Lecce, Italy Anisotropic colloidal nanocrystals as ultrastable polarized quantum emitters P57 Kelly-Ann Vere, Joanna Richens and Paul O’Shea Cell Biophysics Group, Institute of Biophysics, Imaging and Optical Science (IBIOS), School of Biology, University of Nottingham, UK NG7 2RD 157 Electrostatic mapping of molecular interactions on the cell surface; identification of novel cell-surface receptor systems OWLS • Genoa, 4-6 July 2012 • Program • Page 23 OWLS Member Assembly July 5th, 2012 in Genoa, Italy (17:30-19:00) Porto Antico Congress Center – Magazzini del Cotone – Scirocco-Libeccio Room Agenda Extra content: Lecture by Francesco Pavone (see program) 1. Discussion of the Agenda 2. President’s Report 3. Update of bylaws 4. Elections ❚❚Board ❙❙ President, Vice-President, Secretary, Treasurer ❙❙ OWLS Representative to ICO ❚❚Regional Council Representatives ❙❙ Africa, North America, South America, Asia, Australia/New Zealand, Europe ❚❚Standing Committees ❙❙ Bylaws and Regulatory Statutes; ❙❙ Technology Transfer, Standardization, and Technology Assessment; ❙❙ Finances; ❙❙ Education and Ethics 5. Meeting schedule ❚❚OWLS 13 Location ❚❚Co-Sponsoring 6. Treasurer’s report and decision of formal approval ❚❚Financial Report 7. New Members 8. Publications 9. Dues 10. Committee Reports 11. Miscellaneous Prof. Alberto Diaspro President of the International Society on Optics Within Life Sciences (OWLS) Page 24 • Program • OWLS • Genoa, 4-6 July 201 2