Profile - Epiconcept
Transkript
Profile - Epiconcept
1. Institution Ondokuz Mayis University, Faculty of Agriculture, Department of Agricultural Biotechnology, Animal Biotechnology Division, 55139, Samsun, Turkey www.omu.edu.tr 2. Principal investigator and contact person Mehmet Kuran 3. Key personnel NAME Mehmet Kuran Mesut Cevik Ugur Sen Alper Kocyigit EMAIL mkuran@omu.edu.tr RESEARCH AREA DETAILS In vitro culture conditions affecting embryo production, metabolism and quality cevikm@omu.edu.tr Influence of growth factors on in vitro embryo production and cryopreservation in cattle, Spermatology ugur.sen@omu.edu.tr In vitro oocyte maturation temperature and embryo development in cattle alper.kocyigit@omu.edu.tr Effects of IGF and LIF on in vitro bovine embryo development 4. Research profile The group has interest in factors influencing in vitro embryo production in cattle and sheep. These factors include culture media components such as IGF, LIF and antioxidants in IVC; and maturation temperature of oocytes and further in vitro developmental capacity of oocytes following IVF from these maturation conditions. The group has also interest in AI and ET in farm animals and preservation of genetic resources in vitro. 5. Key technologies and tools The lab has very recently been established and the culture system is still being evaluated using various known factors affecting in vitro embryo production. Therefore the basics of in vitro embryo production of cattle and sheep embryos are available in the lab presently (e.g. IVM, IVF, IVC, cryopreservation, differential staining). We are planning to develop embryo metabolism monitoring systems in the lab. 6. Selected publications (max. 5) Cevik, M., Sen, U., Kocyigit, A., Soydan, E., Kuran, M. (2011). Development of an In Vitro Bovine Oocyte Maturation System: Effects of Serum, Hormones and Epidermal Growth Factor on Oocyte Maturation. Journal of the Faculty of Veterinary Medicine, University of Kafkas, 17 (6): 1009-1014. Cevik, M., Tas, A., Akkoc, T., Bagis, H. ve Arat, S. (2009). A Comparative Study of Parthenogenetic Activation and In Vitro Fertilization of In Vitro Matured Bovine Oocytes. Turkish Journal of Veterinary and Animal Science, 33 (3): 393-399. Kuran, M, Robinson, JJ, Brown, DS, and McEvoy, TG. 2002. Development, amino acid utilisation and cell allocation in bovine embryos following in vitro production in contrasting culture systems. Reproduction 124: 155-165. Kuran, M., Robinson, J.J., Staines, M.E., and McEvoy, T.G., 2001. Development and de novo protein synthetic activity of bovine embryos produced in vitro in different culture systems. Theriogenology 55: 593-606 Ocak, N., Cam, MA., Kuran, M., 2006. The influence of pre- and post-mating protein supplementation on reproductive performance in ewes maintained on rangeland. Small Ruminant Research, 64: 16-21.