Ayhan Çelik - biocat.arel.edu.tr
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Ayhan Çelik - biocat.arel.edu.tr
Ayhan Çelik (Associate Professor of Biochemistry) Gebze Institute of Technology Department of Chemistry Muallimkoy Campus, P.O. Box: 141 41400, Gebze, Kocaeli, TURKEY www.turqase.com ayhancelik@gyte.edu.tr Tel: +90 262 605 3124 Fax: +90 262 605 3101 DOB: 30 Jan 1973 Education 1997 - 2001 PhD. in Bio-Organic Chemistry, University of Leicester, UK • Title: Utilising Heam Enzymes in Catalytic Oxidation Chemistry (With Prof. Paul M. Cullis and Dr. Emma L. Raven) • Project: Studied on the exploration of potential applications of metalloenzymes in biotransformations and other industrially relevant processes. In particular, two enzyme systems were studied; cytosolic pea ascorbate peroxidase and cytochrome P450 from Bacillus megaterium. 1995 - 1996 MSc. in Analytical Science, University of Hull, UK • Broad based degree, encompassing study of chromatography (e.g. HPLC, GC, and CE), spectroscopy (e.g. Uv-vis, IR, AA, AE, NMR, and SEM), spectrometry (e.g. MS, LC-MS, GCMS) and electroanalytical chemistry (e.g. voltametric methods). (With Prof. Alan Townshend and Dr.S. Lancaster (BP Chemicals) • Project: Research project involved the development of a flow injection (FI) procedure for the determination of impurities in products of organic acids. Significant improvement was achieved over the existing method in terms of accuracy, sensitivity and sample’s numbers (40-fold). The method is exploited by BP Chemicals, Hull. 1989 – 1993 TURKEY BSc. in Chemistry (1st Class Hons.), Karadeniz Technical University, Professional Experience 2008Associate Professor, Department of Chemistry, Gebze Institute of Technology, GEBZE, TURKEY 2007- 2008 Assistant Professor, Department of Chemistry, Gebze Institute of Technology, GEBZE, TURKEY 2005 – 2007 Research Associate, Department of Chemistry, University of Oxford, OXFORD, UK • Researched on a feasibility study into the formic acid economy: Bio-catalytic approach 2002 – 2005 Research Associate-Research Fellow, School of Chemistry, University of Edinburgh, EDINBURGH, UK • • Researched on “Deracemisation processes for the manufacture of chiral sulfoxides using DMSOR from R. capsulatus”. Techniques used including cloning, screening, protein expression and purification together with conventional synthesis methods. Directed evolution biotechnology to develop oxidative enzyme processes. Methods used for an efficient biocatalyst generation including; saturation / random mutagenesis on cytochrome P450 enzymes, development of novel HTS methods. 2000 – 2002 Research Assistant, School of Chemistry, University of Edinburgh, EDINBURGH, UK • Research focused on cytochrome P450 Enzymes, derived from Actinomycetes, as an efficient hydroxylation biocatalysts. Whole-cell and purified enzyme systems were studied for fine chemicals synthesis. Administrative Duties 2012- Faculty board member, Gebze Institute of Technology,GEBZE, TURKEY 2010-2012 Advisory board member of Consistent Education Appliance and Research Center, Gebze Institute of Technology, GEBZE, TURKEY Scientific Duties Editorial Board Member • • • Journal of Applied Biopharmaceutics and Pharmacokinetics, OMICS-Cloning & Transgensis Turkish Journal of Biology Referee • • • Journal of Molecular catalysis B: Enzymatic Biotechnology Progress (ACS journal), Turkish Journal of Chemistry Awards • • • Awarded a scholarship from Turkish Ministry of Education for studying PhD (1997). Awarded a scholarship from Turkish Ministry of Education for studying MSc. (1995) Awarded a prize due to ranking as the first from the top among the students who graduated from KTU, Chemistry Department (1993). Membership • Associate Member of The Royal Society of Chemistry (AMRSC) (2001-2006) • Member of Biotechnology Society of Turkey (2009-) IT Skills/Other Training • • • • Extensive knowledge of Microsoft Office programs. Proficient in a range of Chemistry and general software packages including ChemDraw, ISIS suite, NMR packages, and Origin, EndNote etc. Certificate in Genetic Modification Work, 2003, University of Edinburgh. Course on Enhancing Writing Skills, 2003, University of Edinburgh Interpretation/Translation, GLS Ltd., (English < -- >Turkish) Research Interest Protein Engineering, Enzyme structure/function relationship, Biocatalysis, Biotransformation, High Throughput Screening (HTS), Enzyme kinetics, Immobilization, Novel assay developments, Synthetic organic chemistry, Analytical chemistry, with view to • Enzymes catalysed reactions of importance in organic synthesis, particularly asymmetric and stereoselective transformations in fine chemical synthesis. • Utilizing directed evolution strategies to improve the properties of enzymes in a targeted manor (e.g. improved enantioselectivity & stability, wider substrate specificity) • Development of novel, high throughput screening methods (HTS) for rapid evaluation and selection of improved biocatalyst. (e.g. product/cofactor monitoring, use of surrogate substrates). • Synthesis and characterisation of derivatives of substrates interested by traditional methods. • Studies on structure/function relationship in engineered enzymes. Key Skills: Protein chemistry, analytical techniques, molecular biology (PCR, Cloning, Directed evolution), cell culture and protein purification, enzyme kinetics (pre-steady state and steady-state kinetics), HTS, synthetic chemistry. Projects • Researcher, ‘Molecular engineering of the NADP+-dependent formate dehydrogenase enzyme by using computational and experimental methods’ (funded by TÜBİTAK,112T538), Budget: 161438 TL Date:2012-2014. • Principal Investigator, ‘Development of Nitroreductase/Prodrug based system for cancer therapy’, (funded by TÜBİTAK,110T754), Budget: 234211 TL, Date:2011-2013 • Researcher, ‘Biotechnological treatment of biowastes for the production of high-added value products’ (funded by G.S.R.T. in Greece and by TUBITAK in Turkey), Project Number: 109Y372 Budget: 225786 TL, Date:2011-2013 • Researcher, ‘Polymeric nanoparticles as an efficient delivery system for phthalocyanine based photosensitizers in Photodynamic Therapy (PDT), (funded by TUBİTAK, 110M010), Budget: 25000, Date:2010-2011 • Researcher, A Feasibility Study into the formic Acid Economy: Biocatalytic approach. University of Oxford, Budget:100000 GBP, Date:2005-2007 • Researcher, ‘Deracemisation processes for the manufacture of chiral sulfoxides using DMSO from R. capsulatus’, University of Edinburgh, Budged: 80000 GBP Date: 2002-2004. • Researcher, ‘Directed evolution biotechnology to develop oxidative enzyme processes’. University of Edinburgh, Budged: 300000 GBP, Date: 2002-2003 Thesis Supervised • 2013. Ph.D. Fatih Aktaş, ‘Coupled enzyme system for chiral alcohol synthesis’ (GIT, Chemistry) • 2011. MSc., Betül Genç ‘Alcohol dehydrogenase-manyetic nanoparticles conjugates for chiral alcohol synthesis’ (GIT, Chemistry) • 2011. MSc. Gülden Aşçı, ‘Development of nitroreductase/prodrug based system for cancer therapy’ (GIT, Chemistry) Teaching Experiences Courses tought at GIT • • • Nanobiotechnology (Chem771) (2008-) Industrial Biocatalysis and Biotransformation (Chem 669) (2008-) Biochemistry Labs (Department of Molecular Biology and Genetics, GIT) (2013-) Tutor for MSc. and PhD. Students; responsible for the day-to-day supervision of project students at Edinburgh University (2001-2005), UK Demonstrator for first and second year organic/inorganic labs at Leicester University (19972000), UK Publications Articles published in refereed journals • Çelik, Ayhan, Cullis, P.M., Lloyd Raven, E. “Catalytic Oxidation of p-Cresol by Ascorbate Peroxidase” Arch. Biochem. Biophy (2000), 373,175. • Çelik, Ayhan; Cullis P.M.; Sutcliffe M.J.; Sangar R.; Raven E.L. “Engineering the Active site of Ascorbate Peroxidase” European J. Biochemistry (2001), 268 (1), 78. • Raven E. L; Çelik, Ayhan; Cullis, P.M; Sangar, R and. Sutcliffe, M. J. Biochem. Soc. Trans, (2001), 29, 105. • Roberts, GA., Çelik, Ayhan. et al.. “A self-sufficient cytochrome P450 with a primary structuralorganisation that includes a flavin domain and a [2Fe2S] redox center” J.Biol.Chem. (2003), 278, 48914 • Ulukanli, Sabri, Çelik Ayhan, “Substrate–controlled diastereoselective aziridination of alkenes using 3-acetoxyaminoquinazolinone in the presence of hexamethyldisilizane.” Tetrahedron Letters (2005), 46(2), 197 • Çelik, Ayhan; Speight, Robert E and. Turner, Nicholas J. “Identification of broad specificity P450CAM variants by primary screening against indole as substrate”, ChemComm.(2005), 29, 3652. • Çelik, Ayhan; Sperandio, Davide; Speight Robert E and. Turner, Nicholas J. “Enantioselective epoxidation of Linolenic acid catalysed by Cytochrome P450 BM3”Organic and Biomolecular Chemistry, (2005), 3(15), 2688. • Karabuga, Semistan; Kazaz, Cavit; Kilic, Hamdullah, Ulukanli, Sabri and Çelik, Ayhan “Imination of sulfoxides using 3-acetoxyaminoquinazolinone as nitrogen source in the presence of hexamethyldisilazane” Tetrahedron Letters (2005), 46 (31), 5225. • Çelik, Ayhan, Flitsch, Sabine L. and Turner, Nicholas J. “Efficient terpene hydroxylating biocatalyst derived from Actinomycetes”, Organic and Biomolecular Chemistry, (2005) (16),2930-2934. • Çelik, Ayhan et al, “Substrate specificity of Cytochrome P450 RhF from Rhodococcus sp”, ChemComm.(2006), 43, 4492. • Demir, Ayhan S.; Talpur, Farah N.; Sopaci, S. Betul; Kohring, Gert-W; Celik, Ayhan, Selective oxidation and reduction reactions with cofactor regeneration mediated by galactitol-, lactate-, and formate dehydrogenases immobilized on magnetic nanoparticles, J. Biotechnol. 2011, 152, 176-183. • Çelik, Ayhan and Yetiş, Gülden, An unusually cold active nitroreductase for prodrug activations,Bioorganic & Medicinal Chemistry, 2012, 20 (11), 3540–3550 • Çelik, Ayhan and Aktaş, Fatih, A new NADH-dependent,zinc containing alcohol dehydrogenase from Bacillus thuringiensis serovar israilensis involved in oxidations of short to medium chain primary alcohols, Journal of Molecular Catalysis B: Enzymatic, ( 2013), 89, 114–121 • Hakan Karaoglu, Derya Yanmis, Fulya AY Sal, Ayhan Celik, Sabriye Canakci, Ali Osman Belduz, Biochemical Characterization of a novel Glucose Isomerase from Anoxybacillus gonensis G2T that displays a high level of activity and thermal stability Journal of Molecular Catalysis B: Enzymatic (in press) Patent • Celik, Ayhan ; Davies, Gideon John ; Ruane, Karen Mary ; Davis, Benjamin Guy, Novel Enzyme, Pub Date:15.04.2010, Pub.No:WO/2010/041055, Int.Appl.No: PCT/GB2009/051320, Int Filig Date:06.10.2009 Book/Book Chapter • (Translation) Instant Notes in Biochemistry 3rd Edition, (Hames D., Hooper N.), Taylor Francis. (Section M: Nitrogen Metabolism,2009), Nobel:1479, ISBN:978-605-395-288-6 Conferances • Tuğba Güngör, Ferah Cömert Önder, Mehmet Ay, Ayhan Çelik, Gülden Aşçı Yetiş, Synthesis of nitro aromatic amide compounds for nitroreductase based cancer therapy, 44th IUPAC- World Chemistry Congres, p0175, 11-16 August 2013, İstanbul-Türkiye • Tuğba Güngör, Ferah Cömert Önder, Mehmet Ay, Ayhan Çelik, Gülden Aşçı Yetiş, Synthesis of nitro aromatic sulfonamide compounds for nitroreductase based cancer therapy, 44th IUPAC- World Chemistry Congres, p0181, 11-16 August 2013, İstanbulTürkiye • Gülden Yetiş, Ayhan Çelik, Mehmet Ay, Tuğba Güngör, Ferah Cömert Önder, Melek Tercan Yavaşoğlu, Merve Kurt, Nitroreductase based pro-drug activation, 44th IUPACWorld Chemistry Congres, p1280, 11-16 August 2013, İstanbul-Türkiye • Gülden Yetiş, Ayhan Çelik, Mehmet Ay, Tuğba Güngör, Ferah Cömert Önder, Melek Tercan Yavaşoğlu, Merve Kurt, A novel ssap-ntrb/pro-drug combination for cancer therapy, 44th IUPAC- World Chemistry Congres, p1281, 11-16 August 2013, İstanbulTürkiye • Mehmet Ay, Ayhan Çelik, Ferah Cömert, Melek Yavaşoğlu, Gülden Yetiş, Synthesis Of Some Prodrug Candidates For Nitroreductase Based Cancer Therapy, 10th International Symposium on PHARMACEUTICAL SCIENCES, P-60, June 26-29, 2012, ANKARA • Tuğba GÜNGÖR, Mehmet AY, Gülden AŞÇI YETİŞ, Ayhan ÇELİK, Kanser Tedavisi İçin Nitroredüktaz/İlaç-Öncü Sistemlerinin Geliştirilmesi, 1.İlaç Kongresi, Antalya, 2013 • Gülden Yetiş, Ayhan Çelik, Mehmet Ay, Tuğba Güngör, Ferah Cömert, Melek Tercan Yavaşoğlu, Kanser Terapilerinde Kullanılmak Üzere Ssap-NtrB/ İlaç-Öncü (Prodrug) Bileşik Birleşiminin Araştırılması, 1.İlaç Kongresi, Antalya, 2013 • İlker Ün, N. Neslihan Bozkurt, Fatih Demirci, Ayhan Çelik, NMR Investigations of the PrismylateDerivative(s) Obtained from Enzymatic Reactions,53rd ENC -Experimental NMR Conference, April 15 - 20, 2012, Miami, Florida (Cambridge isotope-The best NMR spectrum Price Winner and Students Stipends Price) • Aksakal F., Can H., Çelik A. “In Silico Prediction of Binding Affinities for CYP102A1Ligand Complexes”. 10. Kimyasal Fizik Kongresi (CPC-X), Ankara, 2012 • Ayhan Celik, N. Saydan, ‘Polymeric nanoparticles as an efficient delivery system for phthalocyanine based photosensitizers in Photodynamic Therapy (PDT)’, 13th IPA WORLD CONGRESS, International Photodynamic Association, May 10-14, 2011, Innsbruck, Austria • G.Asci, M.Göksel, Ayhan Celik, N.Saydan Intracellular Photodynamic Therapy with Novel Photosensitisers Loaded Nanoparticule, 13th IPA WORLD CONGRESS, International Photodynamic Association, May 10-14, 2011, Innsbruck, Austria • Fatih Aktaş, Ayhan Çelik Bacillus thuringiensis serovar israelensis Bakterisinden Alkol Dehidrogenaz Enziminin Klonlanması, Ekspresyonu ve Karakterizasyonu, 25. Ulusal Kimya Kongresi, Erzurum, 2011 (Poster Price) • Betül Genç, Ayhan Çelik, Manyetik Nanopartikül Bağlı İkili Enzim Sistemi ile Kiral Alkol Üretimi, 25. Ulusal Kimya Kongresi, Erzurum, 2011 (Poster Price) • Betül Genç, Gülden Aşçı, Ayhan Çelik, Nitroredüktaz Aktivitesinin Kolay ve Hassas Tayini, 25. Ulusal Kimya Kongresi, Erzurum, 2011 • N.Neslihan Gezer, Ayhan Çelik, One-pot Enzymatic Synthesis of Styrene oxide, 25. Ulusal Kimya Kongresi, Erzurum, 2011 • Pınar Akkuş, Ayhan Çelik,Kofaktör Geri Dönüşümleri için Tüm Hücre Uygulamaları ve Enzimatik Sistemlerin Karşılaştırılması, 25. Ulusal Kimya Kongresi, Erzurum, 2011 • Aişe Ünlü, Aziz Tanrıseven, Ayhan Çelik, Staphylococcus Aureus İzolatlarından Endüstriyel Lipaz Eldesinin Araştırılması, 25. Ulusal Kimya Kongresi, Erzurum, 2011 • Özden Doruköz, Gülden Aşçı, Ayhan Çelik, Naciye Öztürk, NTR Enziminin Elektron Transfer Mekanizmasının Elektrokimyasal Yöntemlerle İncelenmesi, 25. Ulusal Kimya Kongresi, Erzurum, 2011 • Demir AS, Talpur FN, Sopacı SB. Kohring GW, Celik A. Affinity Magnetic Nanoparticle Supported Galactitol Dehydrogenase th Reduction Reactions. 6 • with NADH Recycling for Enantioselective Nonoscience and Nanotechnology Conference, İzmir, 2010 Aşçı G., Saydan N., Çelik A. “Kanser Terapisinde Kullanılan Nitroredüktaz/İlaç-öncü (prodrug) Sistemlerinin Geliştirilmesi” 24. Ulusal Kimya Kongresi, Zonguldak, 29 Haziran - 2 Temmuz 2010. • Genç B., Çelik A. “Kiral alkol Sentezine Yönelik Alkol Dehidrogenaz Merkezli Sistemlerin Geliştirilmesi” 24. Ulusal Kimya Kongresi, Zonguldak, 29 Haziran - 2 Temmuz 2010. • Ertan F., Çelik A. “Staphylococcus simulans Kaynaklı Lysostaphin Enziminin Ekspresyonu ve Saflaştırılması” 24. Ulusal Kimya Kongresi, Zonguldak, 29 Haziran - 2 Temmuz 2010. • Aşçı G., Yıkılmaz E., Saydan N., Çelik A. “Fotodinamik Terapide Kullanılmak Amacına Yönelik Fotosensitayzır Yüklü PLGA Nanopartiküllerin Hazırlanması ve Kanser Hücreleri Üzerindeki Etkilerinin Araştırılması” 16. Ulusal Biyoteknoloji Kongresi, Antalya, 13-16 Aralık 2009 • Bozkurt N. Çelik A. “Endüstriyel Prosesler İçin Siklohekzanon Monooksijenaz (CHMO) Enziminin Geliştirilmesi” 16. Ulusal Biyoteknoloji Kongresi, Antalya, 13-16 Aralık 2009. • Aişe Ünlü, Aziz Tanrıseven, Ayhan Çelik, Staphylococcus saprophytıcus lipaz için rekombinant sistem geliştirilmesi, 23. Ulusal Kimya Kongresi, Sivas, 2009 (Poster Price) • Pınar Akkuş ve Ayhan Çelik, Enzimatik İndigo Sentezi, 23. Ulusal Kimya Kongresi, Sivas, 2009 • Çelik, Ayhan; Cullis, P.M., Lloyd Raven, E. “Ascorbate Peroxidase Catalyzed Phenols Oxidation: p-Cresol Oxidation”, RSC Perkin Division–Bioorganic Symposium, 1999, University of Leicester, Leicester, UK • Çelik, Ayhan; Raven, E.L.; Cullis, P.M. “Ascorbate Peroxidase as an Enantio-selective Catalyst in Sulphoxidation”, IBDG Meeting, 2000, London, UK • Çelik Ayhan; Cullis P.M.; Sutcliffe M.J.; Sangar R.; Raven E.L. “Redesign of Ascorbate Peroxidase”, Inorganic Chemistry Symposium, 2000, Wolverhampton Science Park, UK • Çelik, Ayhan “Tailoring Enzymes for Environmentally Benign Oxidation Reactions”, Green Chemistry Symposium, 2000, University of Leicester, UK • Çelik, Ayhan; Cullis, Paul M; Sutcliffe, Michael J; Sangar, Rajesh and Raven, Emma Lloyd, “Molecular redesign of the ascorbate peroxidase active site: enantioselective sulphoxidation of thioethers by the wild type enzyme and its W41A variant” 5th European Biological InorganicChemistry Conference, 2000, Toulouse, France. • Hussain, Haitham; Çelik, Ayhan; Ward, J; Turner, N. “Efficient Hydroxylation Catalysts based upon P450 Enzymes Derived from Actinomycetes”. 12th International Conference on Cytochrome P450, La Grande Motte 2001, France • Çelik, Ayhan; Turner, Nicholas J.; Flitsch, Sabine L.; “Terpene Hydroxylation catalyst based on cytochrome P450 enzymes”, 18th Turkish national congress of Chemistry, 2004, Kars, Turkey. • Roberts, Gareth A; Hunter, Dominic J.B; Çelik, Ayhan; Chapman, Stephen K; Turner, Nicholas J. Flitsch, Sabine L. “A self-sufficient P450 with an FeS center”, 7th International Symposium on Cytochrome P450: Biodiversity and Biotechnology, 2004, Hyogo, Japan. • Çelik, Ayhan, “The roles of Cytochrome P450 enzymes in production of fine chemicals”, 14th Biotechnology Congress, Eskisehir, 2005,Turkey. • Çelik, Ayhan, 'A biocatalytic approach to the formic acid economy', CBS Meeting, Oxford University, UK, 2006 Invited Talks • Ayhan Çelik, Application of Nitroreductases in Enzyme/Prodrug Therapy, 26. Ulusal Kimya Kongresi, Mugla, 1-6 Ekim 2012 • Ayhan Çelik, Engineering Cytochrome P450 BM3 and Formate Dehydrogenase (FDH) for Production of Human Drugs’ Metabolites, 1st Int. Symposium on Sec. Metabolites, Chemical, Biological and Biotechnological Properties, September 12-15, 2011 / Denizli, Turkey • Ayhan Çelik, Multienzymes-magnetic iron oxide nanoparticles hybrid systems, 25. Ulusal Kimya Kongresi, Erzurum, 2011 • Ayhan Çelik, Mass Spectrometry of Proteins and Peptides, İU, ÇAPA Tıp Fakültesi, 14 Nisan 2010 • Ayhan Çelik, Monooksigenaz Enzimleri ve Edüstriyel Uygulamaları, Eskişehir OGÜ, 23 Ekim 2009 • Ayhan Çelik, İnce Kimyasalların sentezi: Enzimatik Biyotransformasyonlar, Anadolu Üniversitesi, Eskişehir, 12 Mart 2009 • Ayhan Çelik, Biyokimyasal Sentezler için NADPH Geridönüşümü, Kongresi, Antalya, 2009 16.Biyoteknoloji Interviews Zaman National Newspaper(2006, with Tuncer Çetinkaya) • • Anadolu Agency (Jan.2013) (Topic: Enzyme-Prodrug Therapy, with Yunus Emre Günaydın) • HaberTurk National TV (Topic: Biochemotheraphy, Akşam Raporu with Ece Güner) (Jan.2013)
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