rtf,l - İzmir Katip Çelebi Üniversitesi
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rtf,l - İzmir Katip Çelebi Üniversitesi
İzmir T.c. Kitip Çetebi Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü TEKL|F isrervıe MEKTUBU Tel: 0 232 329 35 İhale No : : 35 Fax:O 232 325 33 55 1 37 8.03.20l6 İlgiliairim BilimselAraştırmaProjeleriKoordinatörlüğü Üniversitemiz Tıp Fakültesi öğretim üyesi Yrd. Doç. Dr. Zeki SOYPAÇACl tarafından yürütülen 2016-TDUlşin Adı : T|PF-0015 numaralı Pauci -İmmun Glomerulonefrit Tanılı Hastaların boOrel< iğne Biyopsilerinde Mtor Yolağının Rolü için 5 kalem malzeme alımı. iı_ciı-i FlRMAyA, idaremizce, 5 Kalem Mal Alımı lşine ait aşağıdaki mal/hizmetin fiyatlarına ihtiyaç vardır. Listedeki kalemlere fiyat vermenizi rica olunur. ooĞnuoııı ıeıvıiıı nirim ., "/rtf,l Sıra No 1 Malzeme / Hizmet Adı LABORATUVAR DiGER MALZEMELERl (mTOR lam bovası-1 adet - 2 3 4 5 Adet 1 Adet 1 Adet 1 Kutu 1 Kutu 3 Açıklama Marka Birim Fiyat (KDV Haric) Toplam TUTAR ) LABORATUVAR DiĞER MALZEMELERl (Mikrotom bıçağı - Miktar ) LABORATUVAR DiĞER MALZEMELERi (PTEN lam boyası-1 adet - ) LABORATUVAR DiGER MALZEMELERi (TGF-beta lam bovası - Birim ) LABORATUVAR DiĞER MALZEMELERl (Lizinli lam Para Birimi; Tl'dir. Fiyatınızı KDV hariç olarak, TL FÜRK Lirası) cinsinden veriniz. 1-Teklifinizi en geç 25.03.2016 SAAT 17:30 a kadar Bilimse| Araştırma Projeleri Koordinatörlüğü, SATIN ALMA birimine teslim ediniz veya fax ile iletiniz. Tekliflerinizi size gönderilen Matbu form üzerine yazmanz (proforma üzerine yazılmaması) rica olunur. 2 - Teklifinizi KDV hariç, birim fiyat olarak veriniz. Teklif mektubu ve teknik şartnamenin her sayfasını kaşeleyip imzalayınız. Varsa markasını mutlaka yazınız. 3 - Doğrudan Temin Usulü ile alım yapılması halinde aşağıdaki şartlar geçerli olacaktır. 3.1 - Teklif mektubunda vermiş olduğunuz fiyatlar, şartnamede aksine bir süre yoksa Otuz (3O) gün süreyle geçerli olacaktır. 3.2 - Taahhüt edilen mal/veya hizmet, ekli şartnamelerde aksine bir süre yoksa tebliğ tarihinden itibaren en 9eç .:,ı ı.](.]:, ] :;, takvim günü içinde ilgili Birime teslim edilecektir. Bu sürenin bitiminde taahhüdün yerine getirilmemesi durumunda idare tek taraflı olarak alımdan vazgeçebilir. 3.3 - Teklif veren firmalar, yukandaki şartlarla birlikte, varsa; teknik şartname ve / veya numunedeki özellikleri aynen kabul etmiş sayılır. 4- Ödeme Şartları: Ödenek Durumuna Ve Faturanln Saymanlığa antikal ediş slraslna göre (tahminen otuz gün içinde) yapllacaktlr. 5- Teklif mektubumuz size ulaştığı takdirde, olumlu ya da olumsuz yazılı olarak geri bildirim yapnız. (Elden veya faks ile) Adres: İzmir Katip Çelebi Üniversitesi Çiğti Ana Yerleşkesi 35620 Çiğli/İzmir - TÜRKiyE iı_oiı_i FIRMANıN; Adı / Ünvanı: Adres: TeI: E1 126RUo Rev E Page 1 of '! SPRrrV6 BloSclENcE Rabbit Anti-Human Transformlng Growth Factor beta1 (TGFB1) Polyclonal Antibody CATALOG El1260 0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. E11262 0.5 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. E11264 1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. E11261 7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/'1% BSA buffer pH 7.6 with less than 0.1% sodium azide. #: lNTENoED usE: CLONE: lMMUNOGEN: lG ISOWPE: EPlTOPE: MOLECULAR WElGHT SPEClES REACTIV|W: DESGRlPTlON: APPLıcATloNs: lHC PROCEDURE: ı" . n' 1.1 Human placenta Stained with anti-TGF B1 antibody For Research Use Only. Not for use in diagnostic procedures. N/A Recombinant protein encoding human TGFP1 aa 177-391 Rabbit lgG Not determined 12.5/39kDa Human (tested). (See www.sorinqbio.com for informatıon on species reactivity predicted by sequence homology.) A total of three members of the TGF beta family, TcFbetal, TGFbeta2 and TGF beta3, have been identified in mammals. TGF betas mediate their activity by high affini§ binding to the type ll receptor transmembrane protein with a cytoplasmic serinepthreonine kinase domain. lmmunohistochemistry (lHC) Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. AntibodyDilution: lfusingtheconcentrateformatofthisproduct,dilutetheantibody1:25.The dilutions are estimates; actual results may differ because of variabili§ in methods and protocols. Antigen Retrieval: Boil tissue section in 1OmM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody lncubation: lncubate for 30 minutes at RT. Stide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization syStem. POSIT|VE CONTROL: Placenta CELLULAR LOGAL|ZAT|ON: SToRAGE & sTABlLlw Cytoplasm, membrane store at 2-8'c. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. lf unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support at spring.tech@ventana.roche.com. wARNlNGs & PRECAUTİONS: 4300 Hacienda Drive Pleasanton, CA 94588 www. sorinobio.com 1. 2. 3. 4. Avoid contact of reagents with eyes and mucous membranes. lf reagents come into contact with sensitive areas, wash with copious amounts of water. This product is harmful if swallowed. Consult local or state authorities with regard to recommended method of disposal. Avoid microbial contamination of reagents. ,§§,§',rrd ,_.,}l,iitı rel: 1-925-474-8440 Toll Free: 1 -800-787-6896 Fax 1-925-474-8469 M470RUo Rev D Page 2 oi 2 WESTERN BLOTT|NG POSlTlVE CONTROL: FLOW CYTOMETRY: FLOW CYTOMETRY MCF-7 Ce|l Lysate Recommended starting protocol: Dilute the antibody 1:400. lncubate for 30 minutes at 4"C. The dilution is an estimate; actual results may differ because of Variability in methods and protocols, optimal dilution and procedure should be determined by the end user. POSIT|VE GONTROL: 4431 cell Line CELLULAR LOCAL|ZAT|ON: Cytoplasm STORAGE & STAB|LITY: wARNlNGs & PRECAUT|ONS: 4300 Hacienda Drive Pleasanton, CA 94588 www. sorinobio.com Store at 2_8'C, Do not freeze, The user must validate any other storage conditions. When properly stored, the reagent is Stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. lf unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support at spring.tech@ventana.roche.com. ,l Avoid contact of reagents with eyes and mucous membranes. lf reagents come into contact with sensitive areas, wash with copious amounts of water. This product is harmful if swallowed. Consult local or stale authorities with regard to recommended method of disposal. Avoid microbial contamination of reagents. . 2. 3. 4. re| 1-925-474-8440 Toll Free: 1-800-787-6896 Fax: 1-925-474-8469 M470RUo Rev D Page 1 ot 2 _*iF{ry.q Rabbit Anti-Human PTEN Monoclonal Antibody (CIone ıD. CATALOG 0.1 ml rabbit monoc|onal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. #: ı@- M4702 0.5 ml rabbit monoclonal antibody purified by protein Aı/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. M4704 1.0 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. adenocarcinoma stained with anti-PTEN antibody M4701 7.0 ml pre-diluted rabbit monoclonal antibody purified by protein A,/G in TBS/1% BSA buffer pH 7.6 with less than 0.1 % sodium azide. : 5:A l\ -,- /\ .l_J___\./ western Blot analysis of MCF-7 cell lysate with PTEN antibody. l\ /\ ^\ _ H:J;r:ff!;;ıi'!;:;,"; antibody in A431 (green) compare to negative control of rabbit lgc (blue) For Research Use Only. Notfor use in diagnostic procedures. lNTENDED USE: CLONE: sP"|70 lMMUNOGEN: lG ISOTYPE: Synthetic peptide close to the N-terminus of human PTEN protein. Rabbit lgG EPlTOPE: MOLECULAR WElGHT: Not determined SPEClES REACT|V|TY: Human (tested). (See www.sprinob|o.com for information on species reactivity predicted by sequence homology.) Phosphatidylinosito|_3,4, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase (PTEN) is a tumor suppressor and a member in the Pl3I(PTEN/AKT pathway. PTEN is a phosphatase with dual specificity to slow cell groMh and antagonize the growth promoting activities from HER protein kinases. The defects of PTEN, mostly around the C{erminus of PTEN, have been implicated in many human cancers and diseases. Therefore, antibody to the N terminus will not be ab|e to detect most of the PTEN protein loss. lmmunohistochemistry (lHC), Western Blotting and Flow cytometry DESCRlPTlON: APPLlGATlONS: lHG PROCEDURE: 54 kDa Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: lf using the concenlrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protoco|s. AntigenRetrieval: Boil tissuesectioninEDTAbuffer,pH8.0forlOminfol|owedbycoolingat room temperature for 20 min. Primary Antibody lncubation: lncubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. lHc PoslTıVE coNTRoL: prostate Adenocarcinoma WESTERN BLOTllNG: Recommended starting protocol: Dilute the antibody 1:25. lncubate for 1 hour at room temperature. The di|ution is an estimate; actual results maydiffer because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. 4300 Hacienda Drive Pleasanton, CA 94588 www.sprinobio.com i}i*' Te| 1-925-474-8440 Toll Free: 1-800-787-6896 Fax: 1-925-474-8469 E1859RUo Rgv E Paga 2 oİ 2 WARNlNGs & PREcAuTloNs: 1, 2, 3. 4. 4300 Hacienda DriVe Pleasanton, cA 94588 www.sorinobio.com Avoid contact of reagents with eyes and mucous mombranos, lf reagents come into contact with sansitive areas, wash with copious amounts of wat€r. This product is harmful if swallowed, consull local or state aı]thorities with regard to recommended method of disposal, Avoid microbial contamination of rcagents, re| |92'47 4-8440 Toll Free: 1-800-787-6896 Fax: 1-92 74-8469 E1859RUo ReV E Paga 1 of 2 -ÇeF{ryg ffi yffi Rabbit Anti-Human mTOR Polyclonal Antibody cATALoG f: E'l8590 0.1 ml ammunogen affinily purifiad labbit polyclonal antibody in PBs/1% BsA buffer pH 7.6 with Iess than 0.1% sodium azide. El8592 o.5 ml immunogen affinity purjfied rabbit polyclonal antibody in PBs/l% BsA buffer pH 7.6 with les§ than 0.1% sodium azide. Et8594 1.o ml immunogen affinity purified rabbit polyclonal antibody in PBs/1% BsA buffer pH 7.6 with less lhan 0.1"/o sodium azide. E18591 7.o ml pre-diluted immunogen atinity pürrified rabbit polyclonal antibody in TBs/1% BsA bufier pH 7.6 with less than 0.1% sodium azide, ,.. For Research Use only. Not for use in diagnostic procedures, cLoNEI N/A Synthetic peplide derived from c-terminus of human mToR protein. Rabbit lgG lG lsoTYPEI EPIToPE: MOLECULAR WElGHT sPEclEs REAcTlv|TY: DEscRlPTloN: APPL|cATloNs: lHc PRocEoURE: '.',-{'^ Human breasI carcinama w ith anti -mTo R antibody lNTENDED usE: lMMUNoGEN: ı , tI Not determined 289kDa Human (lested). (See u4^4!§grj!gho.ç9E for information on species reactivity predicted by sequence homology,) mToR, o. FKBP12 rapamycin associated protein (FRAP), is one of a family of proteins involved in cellcycle progression, DNA reoombination, and DNA damage deteclion. ln ral, it is a 289kDa prolein (symb;lized RAFTl) wilh significant homology to ihe saccharomyces ce,evisiae protein ioR1 a;; has been shown |o associate with the imrnunophilin FKBP12 in a rapamycin dependent fashion, The mToR-rapamycin complex is known io inhibit progression through the G1 cell cyc]e jn several cell stage by interfering wit'h miiogenic signaling palhways involved in G1 progression ıypÖs, js well as in yeast, Th; binding of FRAP to FKBP12,rapamycin correlate with the abi|ity of these ligands to inhibit cellcycle progression. (lHc) specimen Preparation: Formalin-fixed, paraffin-embeddod tissı]es are suitab|e for use with ihls lmmunohistochemistry primary antibody. beparifiinızation: Deparaffinize slides using xylene or xylene alternative and graded alcohols, Aniiuoay oilution, lf ıJsing lhe concentrate fomat of this product, dilute the antibody 1:50. The dilulions;re estimates; actual results may diffel because of Variability in methods and plotocols, Antigon Retİieval: Boil tissue section in 1omM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody lncubation: lnclbate for 30 minltes at room temperature. slido tİashlng: stldes must b€ washed in between steps. Rinse slides with PBs/o.05% Tween. by the instructions provided with lhe visualization system. Breast carcinoma Membrane, cyloplasm slore at 2-8'C. Do not freeze. The user must Validate any other storage conditions. when ploperly stored, the reagent is slable to lhe date indicated on the label. Do not use lhe reagent beyond the visualizatlona Detect the antibody as instructed PoslTlvE coNTRoL: cELLuLAR LocALlzATloN: sToRAGE & sTABlLlTY oxpiration date. There are no derlnilive signs lo indıcate instability ofthis product; thelefore, positive and negalive controls should be tested simultaneously wilh unknown specimens. lf unexpected results are observed which cannot be explained by Varialions in laboralory procedures and a problem with the reagent rs suspected, conlacl Technical support aı spring.tech@Ventana.roche.com. 4300 Hacienda Drive Pleasanlon, cA 94588 www.sorinobio.com re| |92ç474-u40 Toll Free: 1-800-787-6896 Fax: 1-92u74-u69